|
Getting your Trinity Audio player ready...
|
An isolated virus is not needed to engineer a protein. But because the SARS-CoV-2 spike protein is functional, wherever the genetic sequence for it came from, it is not a laboratory experiment mishap – it is by design.
Let’s not lose touch…Your Government and Big Tech are actively trying to censor the information reported by The Exposé to serve their own needs. Subscribe now to make sure you receive the latest uncensored news in your inbox…
Dr. Sabine Stebel is a molecular biologist and expert in protein design. She obtained a PhD from the genetics department of the University of Freiburg in the field of directed protein evolution and DNA fragmentation with method development of nucleotide exchange and excision technology (“NExT”) DNA shuffling. This field is now called protein engineering. Although it is claimed that protein engineering and gain-of-function are distinct concepts in the field of biotechnology, the difference is in the researcher’s intent rather than the process.
Protein engineering is a method used to modify or design proteins to achieve a specific function or property. This is done by manipulating the protein’s sequence, structure, or function to create a new or improved protein. Protein engineering can be used to create proteins with novel functions, enhance existing functions, or improve protein stability and expression.
Gain-of-function research refers to the acquisition of a new or enhanced function by a protein, gene, or organism. This can occur through various mechanisms, including mutations, gene editing, or protein engineering. Gain-of-function can be beneficial, such as in the development of new enzymes or therapeutic proteins, or detrimental, such as in the creation of pathogens with enhanced virulence.
Dr. Stebel has presented her research on protein design, particularly the SARS-CoV-2 spike protein, and has written about covid-related topics on her Substack page ‘DrBine’s Newsletter’. Her expertise in protein engineering and directed evolution is unique and valuable in understanding the molecular biology of covid-19.
At the end of last month, Stebel joined Doc Malik to discuss almost everything you need to know about the spike protein and protein engineering.
If you are unable to watch the video above on Rumble, you can watch it on Doc Malik’s Substack page HERE which also has an option to view the rough transcript.
During the 1 hour and 40-minute interview, Dr. Stebel manages to pack in a lot of information. We have limited this article to the first half hour or so where she explained how proteins, such as the spike protein, are made and whether it came from a virus. At the end of this article, we have shown a rough chapter and timestamp for topics she discussed after the first 30 minutes.
Having completed her PhD in the field of protein engineering, Dr. Stebel knows how proteins are made in a laboratory.
“Before you start to change a protein, you should know how the wild type works and looks like,” she told Doc Malik. “And they didn’t know that [when designing the protein for the covid injections].”
A protein is a 3-dimensional (“3D”) structure. If it is not known what the wild type of, for example, the spike protein of a coronavirus, looks like then it cannot be known if the coronavirus spike protein that is made in the laboratory is the same thing. In a presentation to Doctors for Covid Ethics, Sr. Stebel used a rhinoceros to illustrate this point.
In the image below on the left is an illustration of a rhino drawn from a description (a genetic code) and on the right is a photo of a rhino (an image of the 3D structure). Although the illustrator did a pretty good job, you can see the two are not the same.
The same has happened with the spike protein. Dr. Stebel explained that they modelled the SARS-CoV-2 spike protein from a genetic code without knowing what the wild-type spike protein looked like:
If you look into the Pfizer data batch on this website that Daily Clout uses, you can see they used a protein file, a PDB [Protein Data Bank] file from a database that was released after the trials started.
So, I have no idea how they modelled [the protein] without a 3D structure. They basically work with a DNA sequence but you can’t predict the 3D structure of a protein based on the sequence. This just doesn’t work, not even with deep learning.
(Note: Deep learning is a subfield of machine learning that uses neural networks to teach computers to process data in a way that is inspired by the human brain. It is a method in artificial intelligence (AI) that allows computers to learn from data without being explicitly programmed.)
Dr. Stebel continued:
In principle, we still don’t know what this protein looks like, because what they did was … microscopy, electron microscopy. They had their unsharp images. They modelled a 3D image based on these photos. And then they started to put in the protein sequence so that it matched the photos.
But they don’t know what it looks like inside. So, you need for that – you need crystal structures, X-ray crystal structures.
And we don’t have any.
Was the SARS-CoV-2 Virus Isolated?
Dr. Stebel doesn’t know. I would really like to know what the truth is, she said. And explained why an isolated virus is not needed to develop a viral gene sequence in a laboratory.
In the context of biology, an artefact is an experimental error or a result that is not actually true. The term is generally used for any result that is not actually true but stems from bad analysis or experimental procedures. As Dr. Stebel explained, an artefact doesn’t produce a working protein so the genetic sequence for the spike protein in the covid injections is not an artefact; it is not due to an experimental error or bad laboratory procedures but has been intentionally designed.
You can reassemble pseudogenes from fragments … You can just cut your gene into tiny little fragments, then reassemble these fragments into a gene. It’s called reassembly PCR, very creative. You can cross over different mutants.
But this also happens if you have different fragments of DNA from wherever, and they can just generate pseudogenes. But these pseudogenes wouldn’t lead to working proteins. This would be too much of a coincidence. I don’t think this protein sequence or this DNA sequence is an artefact. Otherwise, you wouldn’t have a working protein that breaks havoc on the body. But we don’t really know where this sequence came from … But I don’t think this gene is an artefact. Otherwise, the protein wouldn’t work.
They definitely made a spike protein, the source of which we don’t know whether there’s a real virus isolated or not. We just know that there was a code. They were given a code.
Directed Protein Evolution and DNA Shuffling
Dr. Stebel was asked whether the sequence could have been designed using gain of function, “where they’ve been playing around with viruses and genetic sequences and have been trying to make things more toxic and dangerous.”
“To optimise a protein, you don’t need the virus,” Dr. Stebel said. “You can just use, for example, phages, which are viruses for bacteria.”
Instead of the normal feed of a phage, you put in the spike protein. And then you can start to mutate it and optimise it and screen it for better binding to ACE2 or whatever without a real dangerous virus.
Then you can take this optimised protein and put it into any virus you want in cell culture. No problem. So, you can screen for optimised proteins on S1 level without any problems. It’s methods from the late 1990s. I did that with my protein. It’s easy. We did that with students in courses.
A bacteriophage, a phage, is a virus which is like a parasite to a bacterium. They only infect bacteria. They only replicate in bacterial cells. They don’t replicate in human cells. And they’re recognised as one of the most abundant biological agents on Earth.
“Normally you take lambda or whatever phage you have in the lab anyway. There are some house animals in the lab you normally use, and there are phage libraries that you can buy from the usual sellers. And then you can just clone in your genes you want to screen with that phage,” Dr. Stebel explained.
You coat some plasticware with a protein of interest, for example, different kinds of ACE2 from different ethnics. Then you have your phage library where you can mutate the feed of the phage with the spike protein via error-prone PCR. You make a PCR of this spike gene and you just add manganese.
[Manganese has been found to increase the error rate in DNA replication, making TAC (DNA polymerase) more error-prone. DNA polymerases are key proteins that catalyse DNA replication and proofread the DNA for any errors as new nucleotides are being incorporated.]
This manganese makes TAC more error-prone; it’s called error-prone PCR. You have lots of single-point mutations in your gene. Then you put the gene back in the phage, and then you can screen if the protein binds better or less to the protein of interest you coated on the plastic wear.
And you do this in some rounds, and then you can gene shuffle – you cut your genes from the library in tiny little pieces, mix them and reassemble them via a reassembly PCR. So, you can get crossover in the test tube and eliminate bad mutations that are detrimental to your protein and accumulate good mutations.
You can do this in a few weeks if the phage is properly set up and displays your protein in the right setup. And then you can do seven to ten rounds of screening in a month. No problem. We did that with students in practical courses with normal libraries we bought to just identify peptides.
But you can do that with proteins. It’s easy. It’s methods from the late 1990s.
Could bacteriophages manufactured with this spike protein, if it was dispersed in some way, cause toxicity and harm to people and cause a pandemic?
Dr. Stebel doesn’t think such a phage would be a problem and wouldn’t cause an epidemic or pandemic. However, “exosomes by the people spiked are a problem because they display the spike protein on their surface. And this could lead to flu-like symptoms,” she said.
Dr. Stebel previously covered the above in more detail during a presentation to Doctors for Covid Ethics. Her presentation covered protein design, particularly the SARS-CoV-2 spike protein, and the mistakes made in constructing this specific protein.
7 February 2024 (23 mins)
If you are unable to watch the video above on Rumble you can watch it on BitChute HERE.
Resources relating to Dr. Stebel’s presentation to Doctors for Covid Ethics:
- Ugur’s borderline stupid ideas in protein design (Introduction and background), Sabine Stebel, 6 July 2023 (Note: “Ugur” in the title refers to Uğur Şahin, a German oncologist and immunologist who is the founder and CEO of BioNTech)
- Ugur’s borderline nonsense ideas in protein design (Part 2), Sabine Stebel, 27 January 2023
- Protein Engineering series of articles by Dr. Sabine Stebel
- Future Science Series: Protein Design by Directed Evolution in modRNA Injections with Dr. Sabine Stebel, Solari Report, 30 August 2023
Moderna and BioNTech Confirm the Virus Was Not Used to Design the Vaccine Spike Protein
Doc Malik recalled Moderna’s CEO Stéphane Bancel saying that he didn’t have the virus and they were designing their vaccine candidates from a computer sequence. “Do you remember hearing that?” he asked Dr. Stebel.
During an interview with Advisory Board in December 2020, Bancel said: “With covid, our team actually designed the vaccine in two days in silico. We never touched the physical virus; we never had a sample of the virus. And we did it in two days.”
It wasn’t only Bancel who confirmed that the covid injection was designed on a computer (in silico) from a genetic sequence, not the virus. BioNTech CEO Uğur Şahin said the same.
“Using the coronavirus’ genetic sequence, which Chinese researchers published on January 11, Sahin designed 10 different candidates on his computer that weekend … Sahin designed that winning candidate in just a few hours,” Business Insider reported in December 2020.
“That’s [also] what Uğur Şahin even writes in his book,” she said. “He downloaded the sequence from a database and worked just with the sequence, and he predicted the 3D structure of the protein based on this DNA sequence, which is impossible. That just doesn’t work.”
There were Olympic games kind of [competition] at my time [in PhD research] where you got the DNA sequence and then you could test your protein structure prediction program against a protein that was crystallised. We knew the structure and could compare how [well our] program could predict the structure of the protein. As far as I know, no one ever got it right.
What Does the Spike Protein Look Like?
What it doesn’t look like is the cartoon-like animations the public was shown by corporate media. Below is a more realistic image of the spike protein.
The white areas in the image of the protein above represent unknown domains. Protein domains are the basic units of proteins that are self-stabilising and fold independently from the rest of the protein chain or structure. Understanding protein domains is essential for understanding the structure and function of proteins, which is crucial for understanding many biological processes and diseases.
“Like white areas on a map, there live dangerous animals or [whatever] they normally write on these maps, there are white areas on the map of the protein,” Dr. Stebel said. Adding, “This was a [Novavax] paper, I don’t know, 2020, 2022. So, we still didn’t know what this protein really looks like with white areas.”
So, no one knows what the SARS-CoV-2 spike protein looks like, she said, and that is still the case now.
It is Not a Trimer Protein as Claimed
In biochemistry, a protein trimer is a macromolecular complex formed by three, usually non-covalently bound, macromolecules like proteins or nucleic acids. It is claimed that the SARS-CoV-2 spike protein is a trimeric protein that plays a crucial role in the virus’s life cycle. The trimeric protein consists of three copies of the spike glycoprotein with two regions known as S1 and S2. The S1 subunit is responsible for receptor binding and the S2 subunit is responsible for membrane fusion.
“The funny thing is they always talk about a trimeric protein, yeah? But this thing makes hexamers and monomers,” Dr. Stebel told Doc Malik.
Monomers can react together with other monomer molecules to form a larger polymer chain or three-dimensional network in a process called polymerisation. As such, monomers are the building blocks of polymers, very large molecules linked together into chains of repeating subunits.
A hexamer is a polymer formed from six molecules of a monomer. In the context of molecular biology, a hexamer is a structural subunit that is part of a viral capsid and is itself composed of six subunits of similar shape and size.
The image below is of a trimer. “A trimer can bind to another trimer and to yet another trimer,” Dr. Stebel explained.
“So [as seen in the image below] you have three trimers bound to each other. [The image is] from the Novavax paper. Okay, so when you say trimer, you mean the protein has three parts to it, yeah? Three subunits. And the three subunits can actually link to create six subunits and a nine-subunit conglomerate,” she said.
Which means it is not a trimer but a hexamer or a polymer. “And that’s from the Novavax paper from 2020, so they knew that,” Dr. Stebel said. In other words they have lied to us, yet again.
Chapter and timestamps for the remainder of Dr. Stebel’s interview with Doc Malik
- 30:58 Are spike proteins always locked onto the cell surface or can they break off into the bloodstream?
- 33:54 How much spike protein does the body produce?
- 35:13 Are spike proteins responsible for the intravascular clots?
- 39:13 In 2020, Stebel was working for a company that processed PCR tests. She quit because the method they were using was unscientific. She explained why. She now writes expert opinions for lawyers on the mechanisms of harm from medicinal products.
- 45:52 What harm can the spike protein cause?
- 47:28 What can people who have had covid injections do to minimise the risk or downplay the action of the spike protein and stop it from producing?
- 53:56 What are the issues with the lipid nanoparticles in the covid injections?
- 1:00:01 Were the vaccines simply people making mistakes or was it deliberate harm and criminality?
- 1:06:40 BioNTech began engineering the protein for its vaccines in January/February 2020 before the covid pandemic had been declared.
- 1:13:52 If you have been vaccinated are you a genetically modified human being?
- 1:16:09 What is driving the people developing and marketing mRNA products?
- 1:17:48 Did certain “elites” receive saline solutions instead of a covid vaccine?
- 1:19:48 What advice would you give?
The Expose Urgently Needs Your Help…
Can you please help to keep the lights on with The Expose’s honest, reliable, powerful and truthful journalism?
Your Government & Big Tech organisations
try to silence & shut down The Expose.
So we need your help to ensure
we can continue to bring you the
facts the mainstream refuses to.
The government does not fund us
to publish lies and propaganda on their
behalf like the Mainstream Media.
Instead, we rely solely on your support. So
please support us in our efforts to bring
you honest, reliable, investigative journalism
today. It’s secure, quick and easy.
Please choose your preferred method below to show your support.
Categories: Breaking News, World News
https://karenkingston.substack.com/p/part-10-mrna-technology-was-invented November 4, 2022. …”It’s critical to note that the patented ‘two proline mutations’ (S-2P) are toxins from synthetically recreated snake venom and the rabies virus.
file:///var/mobile/Library/SMS/Attachments/fc/12/B3FBA792-C379-447C-9E1C-52892AE2AACC/6117891a-07f0-4f6f-9f38-5ba749d43aed_955x541.png.webp
The toxins in the FDA-approved COMIRNATY vaccines are classified as neurotoxins. Per Professor James Giordano, advisor to DARPA and the US Intelligence Community through his organization HDIAC, Krait snake toxin and Cobra toxins are classified as neuroweapons.
file:///var/mobile/Library/SMS/Attachments/a7/07/66F2C215-BA71-45A9-A6FF-7688C7DEB07F/6fc22dfc-aac4-45e3-92b5-90550c3564bf_956x541.png.webp
James Giordano writes that, ‘neuroweapons include drugs to degrade physiologic and cognitive functions, and/or to alter emotional states to affect the desire or capacity for aggression and combat; organic toxins that can induce neuromuscular paralysis and death;”
Giordano clearly states that drugs (or ‘vaccines’) can be used to reduce physical and cognitive abilities and increase or decrease various states of emotions, including capacity for aggression or combat. If you wonder, “Why Didn’t the Americans Unite to Save Humanity,” this is your answer.
Giordano goes on to state that, “microbial agents (e.g., bacteria and viruses – inclusive of “bio-hacked”, genetically-modified organisms) that can incur various levels of morbidity – or mortality, and a number of technologies that can be used to alter sensory, perceptual, cognitive and motor functions.”
James Giordano clearly states that biohacked, genetically modified TECHNOLOGY-ORGANISMS may be referred to as VIRUSES. The SARS-CoV-2 virus, is not a virus. SAR-CoV-2 is an Ai parasite that is a weapon of mass destruction, part biology part technology (Ai).
These Ai bioweapons are in all mRNA COVID-19 vaccines. The bioweapons are deceptively referred to as spike proteins, lipids, and mRNA, i.e., “COMIRNATY contains 30 mcg of mRNA encoding the viral (S) glycoprotein (spike protein) of SARS-CoV-2.”
“Part 10: mRNA Technology was Invented to Benefit Humanity is a Deceptive Claim
Dismantling COVID-19 Deceptions: mRNA technology was created by men who want to be gods by ‘writing DNA,’ the code of life”
KAREN KINGSTON November 2022 Pfizer’s Patent Docs
https://www.rumormillnews.com/cgi-bin/forum.cgi?read=243188
Anthony Fauci stated in a “national (internal) Covid update press conference frim the White House: “It’s a computer model” video scrubbed from internet, or can you find it?
https://www.rumormillnews.com/cgi-bin/forum.cgi?read=243188
Ask Moderna: Let’s start by looking at the confidential agreement proving Moderna had a Coronavirus vaccine candidate at least nineteen days before the alleged emergence of SARS-CoV-2 in Wuhan, China.
The confidential agreement states that providers ‘Moderna’ alongside the ‘National Institute of Allergy and Infectious Diseases’ (NIAID) agreed to transfer ‘mRNA coronavirus vaccine candidates’ developed and jointly-owned by NIAID and Moderna to recipients ‘The University of North Carolina at Chapel Hill’ on the 12th December 2019.
The World Health Organisation declared COVID‑19 a pandemic on 11 March 2020
On February 23 the Daily Mail ran an article showing that Moderna has patented the 19 base letter (nucleotide) sequence which codes for the Furin Cleavage site in Covid-19.
However, research shows that Moderna did not merely apply for a patent in 2016 with US9587003B2: as reported in the Daily Mail. They actually applied in 2013 for 4 patents with US9149506B2, US9216205B2, US9255129B2, US9301993B2, as well for their “Covid-19 virus”.
The final codon completed inserted gene sequence, ‘CTCCTCGGCGGGCA’, patented by Moderna, does not exist in natural viruses and neither does the CGG-coded Furin Cleavage site CCTCGGCGGGCACGT.
Moderna wins Covid-19 shot patent case against Pfizer-BioNTech in Europe May 18, 2024, 07:01 PM Pfizer-BioNTech who used Moderna Virus 2013: #CTCCTCGGCGGGCACGTAG to make their vaccine from.
And you got suckered into having a ModRNA DNA made in a laboratory, synthetic, patented vaccine, which changes your Human Genome and DNA in 6 hours to Trans Human, by US Supreme Court Law with World Wide Applications (2013) – no longer legally Human.
[…] Related: Dr. Sabine Stebel: A virus does not need to be isolated to engineer a viral protein […]
[…] Înrudit: Dr. Sabine Stebel: Un virus nu trebuie izolat pentru a crea o proteină virală […]
[…] Related: Dr. Sabine Stebel: A virus does not need to be isolated to engineer a viral protein […]
[…] Înrudite: Dr. Sabine Stebel: Un virus nu trebuie să fie izolat pentru a inginerie o proteină virală […]
[…] Related: Dr. Sabine Stebel: A virus does not need to be isolated to engineer a viral protein […]
OK. Here we go again. I AM so glad to see all of this info finally coming out, even if just to the “intelligencia”. Hopefully it will filter down to the “masses” soon enough to actually cause a giant disruption of PHARMA.
ONE OF THE PRIMARY ISSUES FLYING BY THE SUPPOSED EXPERTS ARE THE ACTUAL PRODUCTION PROCESSES. WHEN RALPH MADE THE “STABILIZED” VARIANTS, THERE WERE 720 POSSIBLE VARIANTS CREATED. THEN FT. DETRICK, FAUCI AND WUHAN, ADDED THE (AT LEAST) FIVE KNOWN GoF’s. TOGETHER THAT MEANS THERE ARE SIX FACTORIAL x FIVE FACTORIAL POSSIBLE VARIANTS (86,400).
THERE IS NOT ONE DOCTOR ON THE PLANET WHO KNOWS WHICH VARIANT YOU HAVE / HAVE HAD, OR WHICH HE IS TREATING AT THE MOMENT. AND EACH JAB CAUSED MORE IMMUNE SYSTEM HARM THAN GOOD. THERE NEVER WAS ANY IMMUNITY CREATED BY ANY OF THEM.
I PERSONALLY POSTED ALL OF MOUSE-SARS-CV-2002 AS MODIFIED TO BIOWEAPON STATUS, 05DEC19. EVERYTHING. WHO, WHAT, WHERE, WHEN, HOW, – WHY? AT MULTIPLE MEDICAL WEB SITES, SEVERAL JOURNAL SITES, AND ON MY FAVORITE FIGHT CLUB SITES.
ABSOLUTELY EVERYTHING WAS GONE THE NEXT DAY, WHEN I GOT A NEW COMPUTER UP. A NEW COMPUTER BECAUSE THE INSTANT I OPENED MY FIRST BROWSER, MY COMP WAS ATTACKED AND DESTROYED. TWO MORE DESTROYED BEFORE XMAS, TRYING TO POST ANYTHING, ANYWHERE. CANCELLED – DELETED – SHADOW BANNED.
16JAN20 I WAS IN THE ER WITH FULL BLOWN MULTI GAIN OF FUNCTION CV. A-FIB AT RAPID RATE AND BP “UNREADABLE/180”, BRAIN FOG, TASTE/SMELL GONE/WEIRD, ETC. GEE, WITH THREE WEEK INCUBATION IN NASAL GANGLIA, THAT MEANS I WAS “GIVEN” THIS BUG JUST AFTER XMAS. I WONDER WHY?? EXPOSING THE PLAN????? DILTIAZEM FOR THE ACE2 INDUCED A-FIB, AND BP, NATTOKINASE FOR THE ACE2 CLOTS AS WELL, I MUST USE IVERMECTIN CONTINUOUSLY OR THE CYCLE WILL ACCELERATE AND WORSEN IN SYMPTOMS. I TRIED TWICE – WON’T EVER AGAIN)
BUT I SURVIVED WHAT WAS CLEARLY INTENDED TO KILL ME (EX MILITARY FIT AND USING SEVERAL NATURAL ITEMS WHICH ARE NOW USED FOR TREATMENT). TWO WEEKS TO BEING GOOD AGAIN.
THEN IT HAPPENED ALL OVER AGAIN. IN JUST EIGHT WEEKS. THE EXACT SAME. WITH THE SAME THERAPY WORKING.
AND EVERY TEN WEEKS FOR FIVE YEARS NOW.! ! ! ! ! !!
IT IS CLEAR THAT MY BUG ALSO CONTAINED THE SV40 TRANSCRIPTION PROMOTER AS WELL, AS THE GENE THERAPY IT IS, DID EXACTLY AS WAS INTENDED.
TRANSCRIBED INTO MY PERSONAL DNA.
I WILL NEVER BE FREE OF THIS BUG (( YES I HAVE ALREADY TRIED SEVERAL OF THE NOW RECOGNIZED AS GENERALLY EFFECTIVE NATURAL/MEDICAL PROTOCOLS. TO NO EFFECT))
ANYONE OUT THERE GOT ANYTHING MORE TO TRY.???? MY BACKGROUND IN HUMAN MOLECULAR GENETICS, SIX DEGREES, AND THREE RESIDENCIES WILL DECIDE WHETHER THE RISK IS RATIONAL.
ANYONE CARE TO EVAL THE OLDEST, LONGEST LIVING, SURVIVOR OF THE FULL FIVE GAINS OF FUNCTION BIOWEAPON CALLED COVID? DROP A LINE.
“It is highly unlikely the updated virus are false”= it is unsure,presumed,an unproven hypothesis,a supposition without proof therefore not a scientific fact.
Contagion has not been proven.
The existence of viruses has never been proven. Isolation,sequencing of this ghost virus has never been undertaken.
Therefore the cause of flue has not been proven. These diseases exist and should be treated as best as we can eventhough the level of our understanding of the cause of the flue is rather limited. Medical science can only cure 20% of illnesses which implies the understanding of illness and health is very poor. 80% of illnesses medical science does not know how to cure.